Synthesis of Deoxy Derivatives of Lactose and their Hydrolysis by beta-Galactosidase from E. Coli.
نویسندگان
چکیده
منابع مشابه
synthesis of sulfides from alcohols and thiols in solvent-freeconditions and deoxygenation of sulfoxides
کاتالیست یک سنتز جدید برای تیواترها توصیف شده است. واکنش الکل ها با آریل، هتروآریل و آلکیل تیو ل ها درحضور 1،3،5- تری آزو- 2،4،6- تری فسفرین-2،2،4،4،6،6 هگزاکلراید ((tapc به عنوان یک کاتالیست موُثر، بازده های خوب تا عالی از تیواترها را حاصل می کند. علاوه براین، واکنش تحت شرایط بدون فلز و بدون حلال پیش می رود، بنابراین یک مکمل جالب برای روش های شناخته شده سنتز تیواترها ارائه می دهد. یک مکانیسم ا...
15 صفحه اولFormation of galacto-oligosaccharides during lactose hydrolysis by a novel beta-galactosidase from the moderately thermophilic fungus Talaromyces thermophilus.
Discontinuous and continuous processes of lactose hydrolysis and concomitant galacto-oligosaccharide (GalOS) formation were studied. To this end a wide experimental range of the main variables was evaluated, including the initial lactose concentration, the degree of lactose conversion, the pH value and the temperature for discontinuous transformations, while the initial lactose concentration an...
متن کاملTransgalactosylation activity of ebg beta-galactosidase synthesizes allolactose from lactose.
ebg enzyme, the second beta-galactosidase of Escherichia coli, does not normally convert lactose into an inducer of the lac operon. We previously reported the existence of a mutant ebg enzyme that does make such an inducer in vivo (Rolseth et al., J. Bacteriol. 142:1036-1039, 1980). Here I report that the mutant enzyme makes inducer from lactose in vitro and that the inducer is allolactose. Al...
متن کاملin-house production of lactose-hydrolysed milk by beta-galactosidase from lactobacillus bulgaricus
crude enzyme (beta-galactosidase) extract (cee) was produced by lactobacillus ssp. bulgaricus chr hansen lb-12 and was applied in sterile milk which had been processed through ultra high temperature method (uht milk), for hydrolyzing lactose. lactosehydrolyzed milk was also produced by a pure and commercially available betagalactosidase (maxilact). optimum quantities of cee and maxilact enz...
متن کاملEnzymatic Synthesis of Galactosylated Serine/Threonine Derivatives by β-Galactosidase from Escherichia coli
The transgalactosylations of serine/threonine derivatives were investigated using β-galactosidase from Escherichia coli as biocatalyst. Using ortho-nitrophenyl-β-D-galactoside as donor, the highest bioconversion yield of transgalactosylated N-carboxy benzyl L-serine benzyl ester (23.2%) was achieved in heptane:buffer medium (70:30), whereas with the lactose, the highest bioconversion yield (3.9...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1992
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.46-0186